Cell · In Press· Donghui Li, Alberto De Iaco, Kevin C. Ngan, Georgy Smyshlyaev, Ninad Kumbhojkar, Barrett E. Steinberg, Zohra Kalani, Lingbo Xia, Alexas Dolbee, Emily S. Kibbler, Miles C. Duncan, Ian Lin, Matthew S. Benczkowski, Lavanya Venkatesan, Andrea Calabria, Thomas A. Carter, Giulia I. Corsi, Trine A. Kristiansen, Gregory McAllister, Christopher Chase Bolt, Kimberly A. Harris, Jeffrey I. Boucher, Chunxi Zeng, Isha S. Dakhara, Xijia Wang, Shanmugapriya Sothiselvam, Ersin Akinci, Justin Strickland, Anubhuti Mathur, Christina Ferren, Jessica Desmond, Jason Rodriguez, James B. Rottman, Mohit Gupta, Lei Liu, Rahul Palchaudhuri, Z. Jane Wang, Robert J. Citorik, Jacob R. Rubens, Narahari S. Pujar, Michael C. Holmes, Cecilia Cotta-Ramusino·· 17 小时前AI 评分42
利用工程化逆转座子进行体内基因写入
In vivo Gene Writing with engineered retrotransposons
AI 导读
工程化非LTR逆转座子Gene Writers可通过全RNA递送在人类细胞中实现转基因稳定整合，并在人源化小鼠模型中用于体内生成功能性CAR-T细胞。研究通过计算重建、筛选和工程改造获得这些元件，并进行了广泛表征与优化。
来源：Cell · In Press · cell.com